live dead cell staining kit  (Yeasen Biotechnology)

Journal: Bioactive Materials

Article Title: Microenvironment-responsive injectable dynamic hydrogel for sequential antioxidant and tissue regeneration therapy of radiation-induced skin injury ☆

doi: 10.1016/j.bioactmat.2026.03.057

Figure Lengend Snippet: The antibacterial activity and biocompatibility of hydrogels. (A) Antibacterial image of hydrogels against MRSA . (B) Antibacterial image of hydrogels against E.coil . (C, D) Relative bacterial viability of MRSA and E.coil after 24 h of co-incubation with the hydrogels. (E)Representative crystal violet-stained images of MRSA and E. coli biofilms after different treatments. (F) Live/Dead staining of L929 cells after co-culture with different hydrogel groups. (G) Hemocompatibility test of different hydrogel groups. (H) CCK8 of L929 cells after co-culture with different hydrogel groups. (*P < 0.05, **P < 0.01, ***P < 0.001, mean ± SD, n = 3).

Article Snippet: The Live/Dead Cell Double Staining Kit was supplied by Jiangsu KeyGEN BioTECH Co., Ltd., and the Cell Counting Kit-8 (CCK-8) was obtained from Beijing Solarbio Science & Technology Co., Ltd.

Techniques: Activity Assay, Incubation, Staining, Co-Culture Assay

Journal: Bioactive Materials

Article Title: Integrated fabrication of a shape-adaptable, antioxidative composite stent for effective closure and biological repair of enteroatmospheric fistula

doi: 10.1016/j.bioactmat.2026.01.014

Figure Lengend Snippet: Biocompatibility and cell migration of the composite stent. (A) Live/dead staining of IEC-6 cells cultured with the stent, showing cell viability. (B) Hemocompatibility of the stent: (i) hemolysis assay of red blood cells, (ii) corresponding hemolysis percentage. (C) Representative images of cell migration in the scratch assay. (D) Quantification of (i) migration area and (ii) percentage closure over time. (E) Representative Transwell images of migrated cells. (F) Quantification of migrated cells in the Transwell assay. Data were presented as mean ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Gel represents the G 2 S 4 hydrogel group, and Comp represents the composite stent group; the same abbreviations are used in the following figures.

Article Snippet: Live/Dead Cell Staining Kit was bought from KeyGEN BioTech (Jiangsu, China).

Techniques: Migration, Staining, Cell Culture, Hemolysis Assay, Wound Healing Assay, Transwell Assay

Journal: Materials Today Bio

Article Title: 3D-printed titanium scaffolds coated with a multifunctional photothermal-responsive hydrogel promote osteoporotic bone defect repair

doi: 10.1016/j.mtbio.2026.102879

Figure Lengend Snippet: Biocompatibility and cell adhesion of the bone marrow mesenchymal stem cells (BMSCs) in different groups. (A) Cell viability at day 1, 4 and 7 via the CCK-8 assay. (B) Percentage of live cells at day 1 assessed by Live/Dead staining. (C) Proportion of adherent BMSCs. (D) Representative fluorescence images of BMSCs obtained by Live/Dead staining. (E) Representative fluorescence images of adherent BMSCs stained with phalloidin. ∗∗ p < 0.01, and ∗∗∗ p < 0.001, n = 3.

Article Snippet: A live/dead cell double-staining kit (KGA9501-100) was obtained from KeyGENBiotech (Nanjing, China), and a CCK-8 assay kit (TM772) was purchased from Dojindo (Tokyo, Japan).

Techniques: CCK-8 Assay, Staining, Fluorescence